Hotspot

trnN-GUU-ndhF

IRb · 110589-111735 · 1147 bp

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Pi 0.00439
MarkerSeek score 47.20
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1147
variable_sites
5
pi
0.00439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CTACCCCTAACGGTCAAAGC AATATATGGGAGGCGGACGA 55.0 50.0 58.0 58.1 1376-1378 bp 1.0 80.1
trnN-GUU-ndhF_p2 ACCTACCCCTAACGGTCAAA AATATATGGGAGGCGGACGA 50.0 50.0 58.0 58.1 1378-1380 bp 1.0 80.0
trnN-GUU-ndhF_p3 ATAGTAACCCCGGTCGGAAT AATATATGGGAGGCGGACGA 50.0 50.0 57.9 58.1 1732-1734 bp 1.0 79.3
trnN-GUU-ndhF_p4 GGTTACCTACCCCTAACGGT AATATATGGGAGGCGGACGA 55.0 50.0 58.1 58.1 1382-1384 bp 1.0 79.1
trnN-GUU-ndhF_p5 CTACCCCTAACGGTCAAAGC TGGAATTACAAACGGGGTCG 55.0 50.0 58.0 58.2 1429-1431 bp 1.0 79.1