Hotspot

petA-psbJ

LSC · 63987-65067 · 1081 bp

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Pi 0.00185
MarkerSeek score 59.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1081
variable_sites
2
pi
0.00185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCCGTTATTTTGGCACA CCGGATTGGGTTCATCTCTG 45.0 55.0 57.4 58.0 1188 bp 1.0 74.6
petA-psbJ_p2 TGCATCCGTTATTTTGGCAC CCGGATTGGGTTCATCTCTG 45.0 55.0 57.4 58.0 1189 bp 1.0 74.6
petA-psbJ_p3 GCATCCGTTATTTTGGCACA TCCGGATTGGGTTCATCTCT 45.0 50.0 57.4 58.1 1189 bp 1.0 74.0
petA-psbJ_p4 TGCATCCGTTATTTTGGCAC TCCGGATTGGGTTCATCTCT 45.0 50.0 57.4 58.1 1190 bp 1.0 74.0
petA-psbJ_p5 CCCAAGGCCTTTTGTTCTTC CCGGATTGGGTTCATCTCTG 50.0 55.0 57.2 58.0 1211 bp 1.0 73.2