Hotspot

psaA-ycf3

LSC · 42211-42841 · 631 bp

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Pi 0.00158
MarkerSeek score 59.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
631
variable_sites
1
pi
0.00158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGTTCTTGAGAAATGGCCGG TTGAAGATCACGAGGCGTTT 50.0 45.0 58.5 57.8 763-764 bp 1.0 75.1
psaA-ycf3_p2 TGTTCTTGAGAAATGGCCGG TTGGTTGAAGATCACGAGGC 50.0 50.0 58.5 58.2 767-768 bp 1.0 74.8
psaA-ycf3_p3 TGTTCTTGAGAAATGGCCGG TGAAGATCACGAGGCGTTTC 50.0 50.0 58.5 58.3 762-763 bp 1.0 74.1
psaA-ycf3_p4 TGTTCTTGAGAAATGGCCGG TCACGAGGCGTTTCGAATAA 50.0 45.0 58.5 57.6 756-757 bp 1.0 73.6
psaA-ycf3_p5 TGTTCTTGAGAAATGGCCGG GCTATAGCGCTTACTCCAGG 50.0 55.0 58.5 57.6 808-809 bp 1.0 73.5