Hotspot

ycf1

SSC · 122364-127691 · 5328 bp

Back to Noccaea

Pi 0.00939

MarkerSeek score 48.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 50
pi: 0.00939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCAAGTTTCTGCAATTTC	CGTTCCCAAAGGCGTAAAAC	40.0	50.0	54.4	58.0	230 bp	1.0	52.7
ycf1_p2	TCCCAAGTTTCTGCAATTTC	TAAAGGATCCATGCGTTCCC	40.0	50.0	54.4	57.7	243 bp	1.0	50.2
ycf1_p3	TCCCAAGTTTCTGCAATTTC	TCCCAAAGGCGTAAAACTGT	40.0	45.0	54.4	57.6	227 bp	1.0	50.2
ycf1_p4	ATCCCAAGTTTCTGCAATTTC	CGTTCCCAAAGGCGTAAAAC	38.1	50.0	55.0	58.0	231 bp	1.0	49.6
ycf1_p5	TCCCAAGTTTCTGCAATTTC	TTCAAGCAAAAGCACACTCG	40.0	45.0	54.4	57.5	193 bp	1.0	49.6