Hotspot

rps16

LSC · 5040-6203 · 1164 bp

Back to Noccaea

Pi 0.0138

MarkerSeek score 52.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1164
variable_sites: 16
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CATGTGTGCGTCAGGGATAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2232-2246 bp	1.0	81.6
rps16_p2	AAATCCAGTCATGTGTGCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	2241-2255 bp	1.0	80.9
rps16_p3	CATGTGTGCGTCAGGGATAA	CTTCTATTGCCTTTGCGCAC	50.0	50.0	58.0	58.1	2199 bp	0.5	59.0
rps16_p4	CATGTGTGCGTCAGGGATAA	CGCACCACCTTTCTCTTTCT	50.0	50.0	58.0	58.1	2184 bp	0.5	58.9
rps16_p5	CATGTGTGCGTCAGGGATAA	TCTTCTATTGCCTTTGCGCA	50.0	45.0	58.0	58.2	2200 bp	0.5	58.4