Hotspot

ndhE

SSC · 116308-116613 · 306 bp

Back to Noccaea

Pi 0.00654

MarkerSeek score 41.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACCCATGCTTCGAGTTGTTT	TTACGTCGGGGCCATAAATG	45.0	50.0	58.0	58.0	1358-1363 bp	1.0	80.6
ndhE_p2	GCTTCGAGTTGTTTCATGCC	TTACGTCGGGGCCATAAATG	50.0	50.0	58.0	58.0	1352-1357 bp	1.0	80.5
ndhE_p3	CGAACCGGCAGATATTGGAA	TTACGTCGGGGCCATAAATG	50.0	50.0	58.1	58.0	1570-1575 bp	1.0	80.2
ndhE_p4	ACCCATGCTTCGAGTTGTTT	TGCCGCACAACTCCTTATTT	45.0	45.0	58.0	58.1	1376-1381 bp	1.0	80.1
ndhE_p5	GCTTCGAGTTGTTTCATGCC	TGCCGCACAACTCCTTATTT	50.0	45.0	58.0	58.1	1370-1375 bp	1.0	80.0