Hotspot

psaC-ndhE

SSC · 116065-116307 · 243 bp

Back to Noccaea

Pi 0.0247

MarkerSeek score 64.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 243
variable_sites: 6
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATACGTCTGTAGGGCATGC	AACTCGCATCAACCAATCGA	50.0	45.0	57.8	57.9	357-362 bp	1.0	78.7
psaC-ndhE_p2	CATGCTCGGACACATTGAGT	AACTCGCATCAACCAATCGA	50.0	45.0	58.3	57.9	342-347 bp	1.0	78.5
psaC-ndhE_p3	AATACGTCTGTAGGGCATGC	CAACTCGCATCAACCAATCG	50.0	50.0	57.8	57.8	358-363 bp	1.0	78.1
psaC-ndhE_p4	CATGCTCGGACACATTGAGT	CAACTCGCATCAACCAATCG	50.0	50.0	58.3	57.8	343-348 bp	1.0	77.9
psaC-ndhE_p5	GCTCGGACACATTGAGTACA	AACTCGCATCAACCAATCGA	50.0	45.0	57.6	57.9	339-344 bp	1.0	77.7