Hotspot

rps11-rpl36

LSC · 77381-77475 · 95 bp

Back to Noccaea

Pi 0.0213

MarkerSeek score 64.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 95
variable_sites: 2
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGAACCACTACGTGTATT	GTTCCAACCCGAGGCATAAA	45.0	50.0	55.5	58.2	186-205 bp	1.0	60.2
rps11-rpl36_p2	CGTGAACCACTACGTGTATT	TTCCAACCCGAGGCATAAAC	45.0	50.0	55.5	58.2	185-204 bp	1.0	60.2
rps11-rpl36_p3	CGTGAACCACTACGTGTATT	AAGTGTCGACTAATCCGCAG	45.0	50.0	55.5	57.7	229-248 bp	1.0	59.1
rps11-rpl36_p4	CGTGAACCACTACGTGTATT	GGCTTCCGTGCGTAAAATTT	45.0	45.0	55.5	57.7	254-273 bp	1.0	58.6
rps11-rpl36_p5	CGTGAACCACTACGTGTATTT	TTCCAACCCGAGGCATAAAC	42.9	50.0	56.3	58.2	185-204 bp	1.0	58.4