Hotspot

rbcL-accD

LSC · 55629-56329 · 701 bp

Back to Noccaea

Pi 0.0158

MarkerSeek score 60.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 11
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	AGCTCGCCTTTGTATTCCAA	55.0	45.0	57.7	57.5	851-852 bp	1.0	75.2
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	AGCTCGCCTTTGTATTCCAA	50.0	45.0	57.5	57.5	838-839 bp	1.0	73.2
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	TGCTTTACTTAGCTCGCCTT	55.0	45.0	57.7	57.2	861-862 bp	1.0	73.0
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TTGCTTTACTTAGCTCGCCT	55.0	45.0	57.7	57.2	862-863 bp	1.0	73.0
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	AGCTCGCCTTTGTATTCCAAT	55.0	42.9	57.7	58.0	851-852 bp	1.0	71.0