Hotspot

psbZ-trnG-GGC

LSC · 35659-36165 · 507 bp

Back to Noccaea

Pi 0.0179

MarkerSeek score 67.80

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 9
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	653-657 bp	1.0	78.4
psbZ-trnG-GGC_p2	CCGTTGTATTTGCGTCTCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	669-673 bp	1.0	78.0
psbZ-trnG-GGC_p3	TTTGCGTCTCCTGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	661-665 bp	1.0	76.4
psbZ-trnG-GGC_p4	ATTTGCGTCTCCTGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	662-666 bp	1.0	76.2
psbZ-trnG-GGC_p5	CGTTGTATTTGCGTCTCCTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	668-672 bp	1.0	73.9