Hotspot

psbK-psbI

LSC · 7252-7654 · 403 bp

Back to Noccaea

Pi 0.0199

MarkerSeek score 64.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 8
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	TTTTATTCTTCGCGTCCCGG	50.0	50.0	57.9	58.6	546-548 bp	1.0	75.8
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	TTTTATTCTTCGCGTCCCGG	45.0	50.0	58.3	58.6	543-545 bp	1.0	73.7
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	TTTTATTCTTCGCGTCCCGG	45.0	50.0	58.3	58.6	544-546 bp	1.0	73.7
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	TTTTATTCTTCGCGTCCCGG	45.0	50.0	58.3	58.6	545-547 bp	1.0	73.7
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	CCGGATTACGTCCTGGATCA	50.0	55.0	57.9	59.0	530-532 bp	1.0	73.2