Hotspot

rps16-trnQ-CAA

LSC · 6204-6653 · 450 bp

Back to Noccaea

Pi 0.0205
MarkerSeek score 55.90
Primer available yes
Label rps16-trnQ-CAA

Feature Metadata

label_name
rps16-trnQ-CAA
feature_type
igs
region
LSC
length_bp
450
variable_sites
9
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-CAA_p1 AAACGCTTGATTCACGCATG GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 885-889 bp 1.0 81.8
rps16-trnQ-CAA_p2 CAAACGCTTGATTCACGCAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 886-890 bp 1.0 81.8
rps16-trnQ-CAA_p3 AAACGCTTGATTCACGCATG CTTACAGCTGCTTGCCAAAC 45.0 50.0 58.0 57.9 1449-1453 bp 1.0 81.1
rps16-trnQ-CAA_p4 CAAACGCTTGATTCACGCAT CTTACAGCTGCTTGCCAAAC 45.0 50.0 58.0 57.9 1450-1454 bp 1.0 81.1
rps16-trnQ-CAA_p5 TTGATTCGCACTAACCCTGG CTTACAGCTGCTTGCCAAAC 50.0 50.0 57.9 57.9 1299 bp 0.5 58.7