Hotspot

trnK-AAA-rps16

LSC · 4371-5039 · 669 bp

Back to Noccaea

Pi 0.0183
MarkerSeek score 58.70
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
669
variable_sites
12
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAATTAAGTCTTAATAAAACGA 50.0 29.2 58.2 53.5 732-738 bp 1.0 42.7
trnK-AAA-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAATTAAGTCTTAATAAAACGA 50.0 29.2 58.2 53.5 731-737 bp 1.0 42.7
trnK-AAA-rps16_p3 AAGCCGAGTACTCTACCGTT CGGAATTAAGTCTTAATAAAACGAA 50.0 28.0 58.2 54.3 731-737 bp 1.0 42.7
trnK-AAA-rps16_p4 AACTCTACCGCAGATCTGGA CGGAATTAAGTCTTAATAAAACGA 50.0 29.2 57.9 53.5 800-806 bp 1.0 42.5
trnK-AAA-rps16_p5 AACTCTACCGCAGATCTGGA CGGAATTAAGTCTTAATAAAACGAA 50.0 28.0 57.9 54.3 800-806 bp 1.0 42.5