Hotspot

rpl16

LSC · 85197-86654 · 1458 bp

Back to Nitraria

Pi 0.0022
MarkerSeek score 60.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1458
variable_sites
8
pi
0.0022
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACCCAAAACTTCTTTCCTTTGG AGCAATTCCACAATTCTATAGGGT 40.9 37.5 57.2 58.0 1548-1558 bp 1.0 40.7
rpl16_p2 CCCAAAACTTCTTTCCTTTGGT AGCAATTCCACAATTCTATAGGGT 40.9 37.5 57.2 58.0 1547-1557 bp 1.0 40.7
rpl16_p3 ACCCAAAACTTCTTTCCTTTGG ACAAAAATGAGCAATTCCACA 40.9 33.3 57.2 54.8 1557-1567 bp 1.0 40.7
rpl16_p4 CCCAAAACTTCTTTCCTTTGGT ACAAAAATGAGCAATTCCACA 40.9 33.3 57.2 54.8 1556-1566 bp 1.0 40.7
rpl16_p5 CCCAAAACTTCTTTCCTTTGGTT AGCAATTCCACAATTCTATAGGGT 39.1 37.5 57.9 58.0 1547-1557 bp 1.0 40.7