Hotspot

trnL-CAA-ycf2

IRa · 149571-151838 · 2268 bp

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Pi 0.000178

MarkerSeek score 50.90

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 2268
variable_sites: 1
pi: 0.000178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	TTCTATAGGAACCGGACGCT	50.0	50.0	58.0	57.9	2479-2564 bp	1.0	82.2
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	TTCTATAGGAACCGGACGCT	50.0	50.0	58.0	57.9	2481-2566 bp	1.0	82.2
trnL-CAA-ycf2_p3	ATGTCCGATTGGGGCCTATA	TTCTATAGGAACCGGACGCT	50.0	50.0	58.0	57.9	2619-2704 bp	1.0	81.8
trnL-CAA-ycf2_p4	TGTCCGATTGGGGCCTATAT	TTCTATAGGAACCGGACGCT	50.0	50.0	58.0	57.9	2618-2703 bp	1.0	81.8
trnL-CAA-ycf2_p5	CCAACACTCCACCTTTGTCA	TTCTATAGGAACCGGACGCT	50.0	50.0	57.9	57.9	2573-2658 bp	1.0	81.7