Hotspot

accD-psaI

LSC · 62877-63532 · 656 bp

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Pi 0.00245

MarkerSeek score 57.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 4
pi: 0.00245
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGACTTTTTGCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	773-779 bp	1.0	78.9
accD-psaI_p2	CAGCTACACGACTTTTTGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	774-780 bp	1.0	78.4
accD-psaI_p3	AGCTACACGACTTTTTGCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.7	57.9	779-785 bp	1.0	78.2
accD-psaI_p4	CAGCTACACGACTTTTTGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.7	57.9	780-786 bp	1.0	77.8
accD-psaI_p5	GCTACACGACTTTTTGCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	772-778 bp	1.0	72.4