Hotspot

rpoB-trnC-GCA

LSC · 28768-30063 · 1296 bp

Back to Nitraria

Pi 0.00534

MarkerSeek score 59.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 16
pi: 0.00534
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1361-1366 bp	1.0	78.4
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1386-1391 bp	1.0	77.4
rpoB-trnC-GCA_p3	GCATTTCCATCTCCGAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1355-1360 bp	1.0	75.3
rpoB-trnC-GCA_p4	ATTCCCGCATTTCCATCTCC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.0	58.8	1398-1403 bp	1.0	75.1
rpoB-trnC-GCA_p5	ACATTCCCGCATTTCCATCT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.6	58.4	1363-1368 bp	1.0	75.0