Hotspot

atpF-atpH

LSC · 14525-15051 · 527 bp

Back to Nitraria

Pi 0.00152

MarkerSeek score 55.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 2
pi: 0.00152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CCTGAAGCGGAGGGAAAAAT	50.0	50.0	57.9	58.2	668-717 bp	1.0	82.0
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGCGGAGGGAAAAATACGAG	50.0	50.0	57.9	57.7	663-712 bp	1.0	80.8
atpF-atpH_p3	CGACTGGCCAGTTAACCAAA	CCTGAAGCGGAGGGAAAAAT	50.0	50.0	58.4	58.2	648-697 bp	1.0	79.8
atpF-atpH_p4	CGACTGGCCAGTTAACCAAA	AGCGGAGGGAAAAATACGAG	50.0	50.0	58.4	57.7	643-692 bp	1.0	78.5
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	661-710 bp	1.0	77.8