Hotspot

trnS-GCU-trnG-UCC

LSC · 9578-10457 · 880 bp

Back to Nitraria

Pi 0.000909

MarkerSeek score 53.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 2
pi: 0.000909
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	941-946 bp	1.0	52.4
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	944-949 bp	1.0	51.3
trnS-GCU-trnG-UCC_p3	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	943-948 bp	1.0	51.3
trnS-GCU-trnG-UCC_p4	AAGATAGCAAGCCATCGACG	ACGAATCACACTTTTACCACT	50.0	38.1	58.1	55.5	1022-1027 bp	1.0	51.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	999-1004 bp	1.0	51.1