Hotspot

psbK-psbI

LSC · 8851-9227 · 377 bp

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Pi 0.00477

MarkerSeek score 48.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 4
pi: 0.00477
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	TTTTATTCTTCACGCCCGGG	55.0	50.0	58.0	58.5	605-609 bp	1.0	76.1
psbK-psbI_p2	TTAGCCAAATTACCCGAGGC	TTTTATTCTTCACGCCCGGG	50.0	50.0	58.0	58.5	609-613 bp	1.0	76.1
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTTTATTCTTCACGCCCGGG	50.0	50.0	57.9	58.5	519-523 bp	1.0	76.1
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTTTATTCTTCACGCCCGGG	45.0	50.0	58.3	58.5	516-520 bp	1.0	74.1
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTTTATTCTTCACGCCCGGG	45.0	50.0	58.3	58.5	517-521 bp	1.0	74.1