Hotspot

rpl16

LSC · 82523-83991 · 1469 bp

Back to Nigella

Pi 0.0188

MarkerSeek score 58.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1469
variable_sites: 88
pi: 0.0188
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GTTAATAGAGGGCAGTCCGC	50.0	55.0	58.0	58.1	2439-2567 bp	0.777778	73.6
rpl16_p2	CGCACCAAAAACTCGAGTTC	AAATTGCAGGGCGTATCGAT	50.0	45.0	58.0	58.0	2200-2225 bp	0.611111	64.8
rpl16_p3	CCCTTTCCTTTCGTTCCACA	AAATTGCAGGGCGTATCGAT	50.0	45.0	58.0	58.0	1801-1823 bp	0.611111	64.7
rpl16_p4	ACCATTGTCACGCTTGAGTT	AAATTGCAGGGCGTATCGAT	45.0	45.0	58.0	58.0	2119-2144 bp	0.611111	64.7
rpl16_p5	ATCGCACCAAAAACTCGAGT	AAATTGCAGGGCGTATCGAT	45.0	45.0	58.1	58.0	2202-2227 bp	0.611111	64.0