Hotspot

ndhG-ndhI

SSC · 118709-119089 · 381 bp

Back to Nigella

Pi 0.0286

MarkerSeek score 62.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 381
variable_sites: 37
pi: 0.0286
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGCCCAGGTAAATCCAT	GGTAACCCGATCGATTCACG	45.0	55.0	56.8	58.5	426-449 bp	1.0	77.6
ndhG-ndhI_p2	GTATTGGCCCAGGTAAATCCA	GGTAACCCGATCGATTCACG	47.6	55.0	57.7	58.5	428-451 bp	1.0	76.5
ndhG-ndhI_p3	TGTATTGGCCCAGGTAAATCC	GGTAACCCGATCGATTCACG	47.6	55.0	57.7	58.5	429-452 bp	1.0	76.5
ndhG-ndhI_p4	AATCATGTATTGGCCCAGGT	GGTAACCCGATCGATTCACG	45.0	55.0	56.8	58.5	437-457 bp	0.944444	75.0
ndhG-ndhI_p5	AAATCATGTATTGGCCCAGGT	GGTAACCCGATCGATTCACG	42.9	55.0	57.6	58.5	438-458 bp	0.944444	73.0