Hotspot

rps12-clpP

LSC · 70826-70990 · 165 bp

Back to Nigella

Pi 0.0354

MarkerSeek score 60.90

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 18
pi: 0.0354
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	AGAGCGGGGGATTTTGTTAC	TGTCACCAGAACAAGCCAAA	50.0	45.0	57.9	57.9	305-313 bp	0.944444	82.6
rps12-clpP_p2	GAGCGGGGGATTTTGTTACA	TGTCACCAGAACAAGCCAAA	50.0	45.0	58.2	57.9	304-312 bp	0.944444	82.3
rps12-clpP_p3	AGAGCGGGGGATTTTGTTAC	ATGTCACCAGAACAAGCCAA	50.0	45.0	57.9	57.6	306-314 bp	0.944444	80.8
rps12-clpP_p4	GAGCGGGGGATTTTGTTACA	ATGTCACCAGAACAAGCCAA	50.0	45.0	58.2	57.6	305-313 bp	0.944444	80.4
rps12-clpP_p5	GAAGAGCGGGGGATTTTGTT	TGTCACCAGAACAAGCCAAA	50.0	45.0	58.5	57.9	307-315 bp	0.944444	79.9