Hotspot

rpl33-rps18

LSC · 68761-68971 · 211 bp

Back to Nigella

Pi 0.0297

MarkerSeek score 65.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 19
pi: 0.0297
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TACGCCTAGTCGATTGGAGT	CAAACGCCTACGAAAAGCTC	50.0	50.0	58.0	57.8	323-353 bp	1.0	86.7
rpl33-rps18_p2	TACGCCTAGTCGATTGGAGT	AATCAATTCGATCCCCCGAC	50.0	50.0	58.0	57.7	354-384 bp	1.0	86.1
rpl33-rps18_p3	CGCCTAGTCGATTGGAGTTG	CAAACGCCTACGAAAAGCTC	55.0	50.0	58.4	57.8	321-351 bp	1.0	83.7
rpl33-rps18_p4	CGCCTAGTCGATTGGAGTTG	AATCAATTCGATCCCCCGAC	55.0	50.0	58.4	57.7	352-382 bp	1.0	83.0
rpl33-rps18_p5	CGACACAATACGCCTAGTCG	CAAACGCCTACGAAAAGCTC	55.0	50.0	58.5	57.8	331-361 bp	1.0	82.7