Hotspot

ndhC-trnV-UAC

LSC · 51303-52389 · 1087 bp

Back to Nigella

Pi 0.0143

MarkerSeek score 62.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 52
pi: 0.0143
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTCGTTAATTGGGGCCAA	AGTGCATGTTGGGTCTTTGA	50.0	45.0	58.4	57.6	1282-1318 bp	1.0	79.0
ndhC-trnV-UAC_p2	TAATTGGGGCCAAAACTCCG	AGTGCATGTTGGGTCTTTGA	50.0	45.0	58.4	57.6	1275-1311 bp	1.0	79.0
ndhC-trnV-UAC_p3	TGGTCCTTCGTTAATTGGGG	AGTGCATGTTGGGTCTTTGA	50.0	45.0	57.5	57.6	1286-1322 bp	1.0	78.6
ndhC-trnV-UAC_p4	CCTTCGTTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGA	50.0	50.0	58.4	57.5	1215-1251 bp	1.0	78.3
ndhC-trnV-UAC_p5	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGA	50.0	50.0	58.4	57.5	1208-1244 bp	1.0	78.3