Hotspot

trnY-GUA-trnE-UUC

LSC · 30966-31033 · 68 bp

Back to Nigella

Pi 0.0444
MarkerSeek score 62.40
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
68
variable_sites
8
pi
0.0444
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.8 58.3 177-200 bp 1.0 81.8
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.8 58.3 178-201 bp 1.0 81.7
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC CTCTCTTTCAAGGAGGCAGC 55.0 55.0 58.4 58.3 156-179 bp 1.0 81.1
trnY-GUA-trnE-UUC_p4 ACGAATTTACAGTCCGTCCC CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.6 58.3 157-180 bp 1.0 80.9
trnY-GUA-trnE-UUC_p5 GCCAACGAATTTACAGTCCG CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.5 58.3 161-184 bp 1.0 79.6