Hotspot

atpF-atpH

LSC · 11773-12250 · 478 bp

Back to Nigella

Pi 0.0264

MarkerSeek score 65.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 40
pi: 0.0264
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TTTGCAAATCCCTTTGTTTAA	50.0	28.6	58.0	52.6	533-568 bp	1.0	49.7
atpF-atpH_p2	AGTGACCCAAGGAAACGAAA	ATTTGCAAATCCCTTTGTTT	45.0	30.0	57.3	52.3	533-568 bp	1.0	49.7
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	ATTTGCAAATCCCTTTGTTT	50.0	30.0	58.0	52.3	534-569 bp	1.0	49.6
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	ATTTGCAAATCCCTTTGTTTA	50.0	28.6	58.0	52.4	534-569 bp	1.0	49.6
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	CTTTTATTTGCAAATCCCTTTGT	50.0	30.4	58.0	54.6	539-574 bp	1.0	49.5