Hotspot

ycf1

SSC · 125790-131483 · 5694 bp

Back to Nicotiana

Pi 0.0116

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5694
variable_sites: 281
pi: 0.0116
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGGTACGCCCTCTTGATA	TGAACAGGCCATAAGAACGG	50.0	50.0	57.8	57.9	822 bp	1.0	84.4
ycf1_p2	TGGTACGCCCTCTTGATAGT	TGAACAGGCCATAAGAACGG	50.0	50.0	57.8	57.9	820 bp	1.0	84.4
ycf1_p3	AACTGTTTTGCGCCTTTGAG	TGAACAGGCCATAAGAACGG	45.0	50.0	57.8	57.9	1027-1057 bp	1.0	83.4
ycf1_p4	AGTCACCCGATTGTTGTTCC	TGAACAGGCCATAAGAACGG	50.0	50.0	58.1	57.9	803 bp	0.933333	81.1
ycf1_p5	CCCTCTTGATAGTCACCCGA	TGAACAGGCCATAAGAACGG	55.0	50.0	58.2	57.9	813 bp	0.933333	80.1