Hotspot

rps15-ycf1

SSC · 125394-125789 · 396 bp

Back to Nicotiana

Pi 0.0224

MarkerSeek score 53.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 396
variable_sites: 35
pi: 0.0224
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCGTTGACGTTTTCCCAA	ATTGTCTCCTGCTTCGACTG	45.0	50.0	58.0	57.6	1230-1245 bp	1.0	81.7
rps15-ycf1_p2	ATGAGTCGAAGAACACCGTG	ATTGTCTCCTGCTTCGACTG	50.0	50.0	57.9	57.6	1512-1527 bp	1.0	81.2
rps15-ycf1_p3	AGTCGTTGACGTTTTCCCAA	TGTCTCCTGCTTCGACTGAT	45.0	50.0	58.0	58.4	1228-1243 bp	1.0	81.2
rps15-ycf1_p4	GCCTAATTGTTCGGGTCCAT	ATTGTCTCCTGCTTCGACTG	50.0	50.0	57.9	57.6	1696-1711 bp	1.0	80.9
rps15-ycf1_p5	GCTAGGAGTCGTTGACGTTT	ATTGTCTCCTGCTTCGACTG	50.0	50.0	57.9	57.6	1236-1251 bp	0.933333	78.2