Hotspot

accD-psaI

LSC · 61204-61960 · 757 bp

Back to Nicotiana

Pi 0.0107

MarkerSeek score 59.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 34
pi: 0.0107
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	932-952 bp	1.0	71.6
accD-psaI_p2	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	931-951 bp	1.0	71.6
accD-psaI_p3	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	917-937 bp	1.0	71.1
accD-psaI_p4	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	47.6	45.0	57.4	57.8	922-942 bp	1.0	71.0
accD-psaI_p5	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.4	57.9	872-892 bp	0.933333	68.2