Hotspot

ndhC-trnV-UAC

LSC · 52657-53757 · 1101 bp

Back to Nicotiana

Pi 0.0154

MarkerSeek score 62.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 71
pi: 0.0154
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTGCTAATCGGGGCTAACAC	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1355-1417 bp	1.0	88.1
ndhC-trnV-UAC_p2	TTGCTAATCGGGGCTAACAC	ATCTAGGCCAGAGACAGACC	50.0	55.0	58.0	57.9	1426-1490 bp	1.0	87.5
ndhC-trnV-UAC_p3	AGGGGGCCATTTTCATTGTT	TGAATCAGAGCACATGGAGC	45.0	50.0	58.0	58.0	1929-1991 bp	1.0	87.0
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	TGAATCAGAGCACATGGAGC	45.0	50.0	58.1	58.0	1964-2026 bp	1.0	86.8
ndhC-trnV-UAC_p5	AGGGGGCCATTTTCATTGTT	ATCTAGGCCAGAGACAGACC	45.0	55.0	58.0	57.9	2000-2064 bp	1.0	86.5