Hotspot

ycf1

SSC · 128743-134409 · 5667 bp

Back to Neviusia

Pi 0.00424

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5667
variable_sites: 24
pi: 0.00424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGAAAAACCGAAGTTCGA	AGTATTGAGGCCTGGATCGA	45.0	50.0	57.7	57.9	228 bp	1.0	77.7
ycf1_p2	TGGGAAAAACCGAAGTTCGA	CAGTATTGAGGCCTGGATCG	45.0	55.0	57.7	57.8	229 bp	1.0	77.1
ycf1_p3	CTGGGAAAAACCGAAGTTCG	AGTATTGAGGCCTGGATCGA	50.0	50.0	57.3	57.9	229 bp	1.0	74.9
ycf1_p4	CTGGGAAAAACCGAAGTTCG	CAGTATTGAGGCCTGGATCG	50.0	55.0	57.3	57.8	230 bp	1.0	74.3
ycf1_p5	GGGAAAAACCGAAGTTCGAG	AGTATTGAGGCCTGGATCGA	50.0	50.0	57.0	57.9	227 bp	1.0	72.6