Hotspot

rpl2

IRa · 158077-159586 · 1510 bp

Back to Neviusia

Pi 0.000662

MarkerSeek score 44.90

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1510
variable_sites: 1
pi: 0.000662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	ACGTGTCACAATTAATTACTCCT	50.0	34.8	58.0	56.0	2116 bp	1.0	40.2
rpl2_p2	GAATGGTTAAAGCGCCCAAC	TGAACGTGTCACAATTAATTACTCC	50.0	36.0	58.0	57.9	2119 bp	1.0	40.2
rpl2_p3	GCATTGGGTCGAACTCTTCT	ACGTGTCACAATTAATTACTCCT	50.0	34.8	57.9	56.0	1782 bp	1.0	40.2
rpl2_p4	GAATGGTTAAAGCGCCCAAC	AATGATTCGCTACAAAAGGA	50.0	35.0	58.0	52.9	2150 bp	1.0	40.1
rpl2_p5	GAATGGTTAAAGCGCCCAAC	TGATTCGCTACAAAAGGATT	50.0	35.0	58.0	52.9	2148 bp	1.0	40.1