Hotspot

rpl23-rpl2

IRa · 158059-158076 · 18 bp

Back to Neviusia

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGCTTCAACCGGGTTATTCT	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	134 bp	1.0	78.3
rpl23-rpl2_p2	CGCTTCAACCGGGTTATTCT	GACTGTCTACGGCTCCATTG	50.0	55.0	58.3	58.1	129 bp	1.0	77.9
rpl23-rpl2_p3	ATCATTACGCTTCAACCGGG	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	141 bp	1.0	77.7
rpl23-rpl2_p4	CGCTTCAACCGGGTTATTCT	ACCACAACGATGCTTTCCAT	50.0	45.0	58.3	58.1	182 bp	1.0	77.6
rpl23-rpl2_p5	ATCATTACGCTTCAACCGGG	GACTGTCTACGGCTCCATTG	50.0	55.0	58.3	58.1	136 bp	1.0	77.3