Hotspot

rpl32-trnL-UAG

SSC · 117490-118917 · 1428 bp

Back to Neviusia

Pi 0.00939

MarkerSeek score 67.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1428
variable_sites: 13
pi: 0.00939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCTTTCTACCGGGAGTTCGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1472-1506 bp	1.0	81.4
rpl32-trnL-UAG_p2	TCTTTCTACCGGGAGTTCGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	1473-1507 bp	1.0	81.3
rpl32-trnL-UAG_p3	ATCGGGCAGCATTAAAAGCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1509-1543 bp	1.0	81.1
rpl32-trnL-UAG_p4	ATCGGGCAGCATTAAAAGCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1510-1544 bp	1.0	81.1
rpl32-trnL-UAG_p5	TCTTTCTACCGGGAGTTCGA	TTAGAAGATGTCATGCCGCC	50.0	50.0	57.8	58.0	1536-1570 bp	1.0	80.9