Hotspot

psaJ-rpl33

LSC · 70414-70889 · 476 bp

Back to Neviusia

Pi 0.00649

MarkerSeek score 62.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 3
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	520-534 bp	1.0	73.3
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	565-579 bp	1.0	73.0
psaJ-rpl33_p3	TGGCACCGGTAGTAAGTACT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	590-604 bp	1.0	72.6
psaJ-rpl33_p4	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	566-580 bp	1.0	72.3
psaJ-rpl33_p5	GTGGCACCGGTAGTAAGTAC	AACATCTTTACCCTTGGCCA	55.0	45.0	57.7	57.0	591-605 bp	1.0	72.1