Hotspot

accD-psaI

LSC · 61993-62794 · 802 bp

Back to Neviusia

Pi 0.00766

MarkerSeek score 65.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 6
pi: 0.00766
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTTTAAAGGGCGTTCTGGGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	940-952 bp	1.0	81.1
accD-psaI_p2	GGCGTTCTGGGTGAGTTATT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	932-944 bp	1.0	81.0
accD-psaI_p3	CTTTAAAGGGCGTTCTGGGT	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.1	58.0	931-943 bp	1.0	81.0
accD-psaI_p4	GGCGTTCTGGGTGAGTTATT	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	923-935 bp	1.0	80.9
accD-psaI_p5	CTTTAAAGGGCGTTCTGGGT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	946-958 bp	1.0	80.5