Hotspot

rbcL-accD

LSC · 59858-60516 · 659 bp

Back to Neviusia

Pi 0.00462

MarkerSeek score 62.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 3
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CCCCCGTTAAACAACATCGA	55.0	50.0	57.7	58.2	766-776 bp	1.0	77.5
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	GAATCGAACCGCCGTTTTTC	55.0	50.0	57.7	58.4	748-758 bp	1.0	75.8
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	CCCCCGTTAAACAACATCGA	50.0	50.0	57.5	58.2	753-763 bp	1.0	75.5
rbcL-accD_p4	CTTGCTCGTGAGGGTAATGA	CCCCCGTTAAACAACATCGA	50.0	50.0	57.3	58.2	810-820 bp	1.0	74.4
rbcL-accD_p5	GCTGCTGCTTGTGAAGTATG	GAATCGAACCGCCGTTTTTC	50.0	50.0	57.5	58.4	735-745 bp	1.0	73.9