Hotspot

trnC-GCA-petN

LSC · 29018-29959 · 942 bp

Back to Neviusia

Pi 0.00425

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 4
pi: 0.00425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	999-1000 bp	1.0	79.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TGCCCCATACCACTAGTGAA	50.0	50.0	58.1	58.0	1047-1048 bp	1.0	79.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCACTTCTGCCC	50.0	55.0	58.1	58.1	1062-1063 bp	1.0	78.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ACTTCTGCCCCATACCACTA	50.0	50.0	58.1	57.7	1052-1053 bp	1.0	77.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTCTGCCCCATACCACTAGT	50.0	50.0	58.1	57.7	1050-1051 bp	1.0	77.5