Hotspot

rps16-trnQ-UUG

LSC · 6540-7493 · 954 bp

Back to Neviusia

Pi 0.00884

MarkerSeek score 60.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 8
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	979-1027 bp	1.0	82.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	990-1038 bp	1.0	82.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTGTCGACTAGAAATGGGGC	45.0	55.0	58.1	58.3	1041-1089 bp	1.0	80.4
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	1052-1100 bp	1.0	79.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	983-1031 bp	1.0	78.1