Hotspot

ndhH-rps15

SSC · 130741-130877 · 137 bp

Back to Neuwiedia

Pi 0.214

MarkerSeek score 89.60

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 137
variable_sites: 25
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	TTCTGGGAAAACGTCAACGA	45.0	45.0	58.1	57.7	319-329 bp	1.0	87.2
ndhH-rps15_p2	AACACCATGCATTGATGGGT	CTGGGAAAACGTCAACGACT	45.0	50.0	58.1	58.4	317-327 bp	1.0	86.4
ndhH-rps15_p3	AACACCATGCATTGATGGGT	TCTGGGAAAACGTCAACGAC	45.0	50.0	58.1	58.4	318-328 bp	1.0	86.3
ndhH-rps15_p4	ATGCATTGATGGGTGGTGAG	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.2	57.7	313-323 bp	1.0	86.0
ndhH-rps15_p5	CATTGATGGGTGGTGAGGAC	TTCTGGGAAAACGTCAACGA	55.0	45.0	58.2	57.7	310-320 bp	1.0	85.9