Hotspot

psbH-petB

LSC · 80601-81323 · 723 bp

Back to Neuwiedia

Pi 0.0154
MarkerSeek score 70.80
Primer available yes
Label psbH-petB

Feature Metadata

label_name
psbH-petB
feature_type
igs
region
LSC
length_bp
723
variable_sites
10
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-petB_p1 GTCGCAATGGCTCTATTTGC TCCGGAATCTCTTCTTTGTGA 50.0 42.9 57.9 56.7 796-871 bp 1.0 65.4
psbH-petB_p2 GTCGCAATGGCTCTATTTGC ACTTCTTGTAGCTTAGCCACT 50.0 42.9 57.9 56.7 848-923 bp 1.0 65.3
psbH-petB_p3 GGAGTCGCAATGGCTCTATT TCCGGAATCTCTTCTTTGTGA 50.0 42.9 57.8 56.7 799-874 bp 1.0 64.4
psbH-petB_p4 GGAGTCGCAATGGCTCTATT ACTTCTTGTAGCTTAGCCACT 50.0 42.9 57.8 56.7 851-926 bp 1.0 64.2
psbH-petB_p5 ACTTATGGGAGTCGCAATGG TCCGGAATCTCTTCTTTGTGA 50.0 42.9 57.7 56.7 806-881 bp 1.0 63.7