Hotspot

petA-psbJ

LSC · 65901-67289 · 1389 bp

Back to Neuwiedia

Pi 0.0206

MarkerSeek score 71.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1389
variable_sites: 24
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGAGTCCAGGGT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.8	58.0	1342-1532 bp	1.0	83.6
petA-psbJ_p2	GTTCTTCTTGGCATCCGTGA	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.2	58.0	1317-1507 bp	1.0	83.5
petA-psbJ_p3	ATCCATTACGAGTCCAGGGT	CTGGGTTGGGTTCATCTCTG	50.0	55.0	57.8	57.9	1341-1531 bp	1.0	83.0
petA-psbJ_p4	CGAGTCCAGGGTCTTTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.7	58.0	1334-1524 bp	1.0	82.9
petA-psbJ_p5	GTTCTTCTTGGCATCCGTGA	CTGGGTTGGGTTCATCTCTG	50.0	55.0	58.2	57.9	1316-1506 bp	1.0	82.9