Hotspot

atpB-rbcL

LSC · 56981-58105 · 1125 bp

Back to Neuwiedia

Pi 0.0641

MarkerSeek score 91.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 64
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 91.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1102-1228 bp	1.0	86.2
atpB-rbcL_p2	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1103-1229 bp	1.0	86.2
atpB-rbcL_p3	GGTGGAAAGGTGACATCGAG	GGTGACATAAGTCCCTCCCT	55.0	55.0	58.3	58.1	1138-1264 bp	1.0	85.1
atpB-rbcL_p4	TGAGTGATACGCCCTAGGTT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	1103-1229 bp	1.0	83.8
atpB-rbcL_p5	TTGAGTGATACGCCCTAGGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	1104-1230 bp	1.0	83.8