Hotspot

petN-psbM

LSC · 30473-31684 · 1212 bp

Back to Neuwiedia

Pi 0.0375

MarkerSeek score 83.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 44
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.2	57.9	1385-1782 bp	1.0	82.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1376-1773 bp	1.0	81.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1377-1774 bp	1.0	80.0
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTGTTCATTT	50.0	45.0	58.2	58.5	1374-1771 bp	1.0	79.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	1375-1772 bp	1.0	79.2