Hotspot

rpl32-trnL(UAG)

SSC · 114448-114989 · 542 bp

Back to Nestegis

Pi 0.00953

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 542
variable_sites: 10
pi: 0.00953
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	669-681 bp	1.0	79.4
rpl32-trnL(UAG)_p2	TGGGACAGCGTTAAAAGCAT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	662-674 bp	1.0	79.0
rpl32-trnL(UAG)_p3	TGGGACAGCGTTAAAAGCAT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.1	57.8	668-680 bp	1.0	78.9
rpl32-trnL(UAG)_p4	TGGGACAGCGTTAAAAGCAT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	667-679 bp	1.0	78.9
rpl32-trnL(UAG)_p5	TGGGACAGCGTTAAAAGCAT	GAGCAGCGTGTCTACCAATT	45.0	50.0	58.1	58.3	663-675 bp	1.0	78.3