Hotspot

rbcL-accD

LSC · 58113-58775 · 663 bp

Back to Nestegis

Pi 0.00377

MarkerSeek score 61.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 5
pi: 0.00377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACCTGCGTTCTAACTCCTTC	55.0	50.0	58.0	57.3	838-846 bp	1.0	74.2
rbcL-accD_p2	ATTTGAGTTTGCCGCAATGG	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	779-787 bp	1.0	73.9
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ACCTGCGTTCTAACTCCTTC	50.0	50.0	57.9	57.3	808-816 bp	1.0	73.6
rbcL-accD_p4	TGAGTTTGCCGCAATGGATA	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	776-784 bp	1.0	73.6
rbcL-accD_p5	AGTTTGCCGCAATGGATACT	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	774-782 bp	1.0	73.5