Hotspot

ycf3-trnS(GGA)

LSC · 45891-46674 · 784 bp

Back to Nestegis

Pi 0.00446

MarkerSeek score 58.30

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 6
pi: 0.00446
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	887 bp	1.0	71.5
ycf3-trnS(GGA)_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	908 bp	1.0	71.0
ycf3-trnS(GGA)_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	858 bp	1.0	70.7
ycf3-trnS(GGA)_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	888 bp	1.0	69.1
ycf3-trnS(GGA)_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	885 bp	1.0	68.4