Hotspot

psbM-trnD(GUC)

LSC · 31025-31669 · 645 bp

Back to Nestegis

Pi 0.00956

MarkerSeek score 41.60

Primer available yes

Label psbM-trnD(GUC)

Feature Metadata

label_name: psbM-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 12
pi: 0.00956
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GUC)_p1	CTGCCGATTATAGTCCGCTC	AGATCCCGCGAGTCTAATCA	55.0	50.0	58.0	58.0	1379 bp	1.0	80.7
psbM-trnD(GUC)_p2	CTGCCGATTATAGTCCGCTC	CCTACGCTGGTTCAAATCCA	55.0	50.0	58.0	57.9	1097 bp	1.0	80.1
psbM-trnD(GUC)_p3	TCATGGTTCGGGCGTTAAAA	AGATCCCGCGAGTCTAATCA	45.0	50.0	58.1	58.0	1886-1934 bp	1.0	79.8
psbM-trnD(GUC)_p4	GGTTCGGGCGTTAAAAATCG	AGATCCCGCGAGTCTAATCA	50.0	50.0	58.1	58.0	1882-1930 bp	1.0	79.7
psbM-trnD(GUC)_p5	GTTCGGGCGTTAAAAATCGG	AGATCCCGCGAGTCTAATCA	50.0	50.0	58.1	58.0	1881-1929 bp	1.0	79.7