Hotspot

petN-psbM

LSC · 29754-30919 · 1166 bp

Back to Nestegis

Pi 0.005

MarkerSeek score 45.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 11
pi: 0.005
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1275-1327 bp	1.0	64.3
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1273-1325 bp	1.0	64.3
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	GCGACTGCACTATTCATTCT	55.0	45.0	57.9	55.9	1272-1324 bp	1.0	63.7
petN-psbM_p4	TAGTGTGGGGAAGAAGTGGA	GCGACTGCACTATTCATTCT	50.0	45.0	57.6	55.9	1270-1322 bp	1.0	61.0
petN-psbM_p5	CCTGGGCTGCTTTAATGGTA	GCGACTGCACTATTCATTCT	50.0	45.0	57.6	55.9	1315-1367 bp	1.0	60.8